5x maxima rt buffer (Thermo Fisher)

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Thermo Fisher 5x maxima rt buffer
5x Maxima Rt Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxima+5x+rt+buffer/5%C3%97+first+strand+buffer/us12163189-208-21-25
Average 90 stars, based on 1 article reviews

5x maxima rt buffer - by Bioz Stars, 2026-11

90/100 stars

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Incubation:

Article Title: Seq-Scope: Submicrometer-resolution spatial transcriptomics for single cell and subcellular studies
Article Snippet: .. After mRNA release, the tissue-attached HDMI array was incubated in 40uL fluorescent reverse transcription solution containing 13 μl water, 8 μl Maxima 5x RT Buffer (EP0751, Thermofisher), 8 μl 20% Ficoll PM-400 (F4375-10G, Sigma), 0.8 μl 100mM dATP (from 0446S, NEB), 0.8 μl 100mM dTTP (from 0446S, NEB), 0.8 μl 100mM dGTP (from 0446S, NEB), 0.1 μl 100mM dCTP (from 0446S, NEB), 1.5 μl 6.45mM Cy3-dCTP (B8159, APExBIO), 1 μl RNase Inhibitor (30281, Lucigen), 4 μl Actinomycin D (500ng/μl, A1410, Sigma-Aldrich) and 2 μl Maxima H- RTase (EP0751, Thermofisher). ..

Article Title: Highly sensitive spatial transcriptomics at near-cellular resolution with Slide-seqV2
Article Snippet: 3) Pre-Alignment: this step runs run_alignment.py, calls the functions of TagBamWithReadSequenceExtended, FilterBam, TrimStartingSequence and PolyATrimmer in the Drop-seq tools ( https://github.com/broadinstitute/Drop-seq ) to tag unmapped bam files with a bead barcode (XC) and a molecular barcode (a.k.a. .. First Strand Synthesis Subsequently, first strand synthesis was performed by incubating the pucks in RT solution for 1.5 hours at 52 C. RT solution: list-behavior=simple prefix-word= mark-type=none max-label-size=0 115 μL H2O 40 μL Maxima 5x RT Buffer (Thermofisher, EP0751) 20 μL 10 mM dNTPs (NEB N0477L) 5 μL RNase Inhibitor (Lucigen 30281) 10 μL 50 μM Template Switch Oligo ( Qiagen #339414YCO0076714) 10 μL Maxima H-RTase (Thermofisher, EP0751) Tissue Digestion: 200 μL of 2x tissue digestion buffer was then added directly to the RT solution and the mixture was incubated at 37C for 30 minutes. .. 2x tissue digestion buffer: 200 mM Tris-Cl pH 8 400 mM NaCl 4% SDS 10 mM EDTA 32 U/mL Proteinase K (NEB P8107S) Second Strand Synthesis: The solution was then pipetted up and down vigorously to remove beads from the surface, and the glass substrate was removed from the tube using forceps and discarded.

Article Title: Highly sensitive spatial transcriptomics at near-cellular resolution with Slide-seqV2
Article Snippet: RNA Hybridization: Pucks in 1.5 mL tubes were immersed in 200 μL of hybridization buffer (6x SSC with 2 U/μL Lucigen NxGen RNAse inhibitor) for 30 minutes at room temperature to allow for binding of the RNA to the oligos on the beads. .. First Strand Synthesis Subsequently, first strand synthesis was performed by incubating the pucks in RT solution for 1.5 hours at 52 C. RT solution: 115 μL H2O 40 μL Maxima 5x RT Buffer (Thermofisher, EP0751) 20 μL 10 mM dNTPs (NEB N0477L) 5 μL RNase Inhibitor (Lucigen 30281) 10 μL 50 μM Template Switch Oligo ( Qiagen #339414YCO0076714) 10 μL Maxima H-RTase (Thermofisher, EP0751) Tissue Digestion: 200 μL of 2x tissue digestion buffer was then added directly to the RT solution and the mixture was incubated at 37C for 30 minutes. .. 2x tissue digestion buffer: 200 mM Tris-Cl pH 8 400 mM NaCl 4% SDS 10 mM EDTA 32 U/mL Proteinase K (NEB P8107S) Second Strand Synthesis: The solution was then pipetted up and down vigorously to remove beads from the surface, and the glass substrate was removed from the tube using forceps and discarded.

Reverse Transcription:

other:

Article Title: Highly sensitive spatial transcriptomics at near-cellular resolution with Slide-seqV2
Article Snippet: Subsequently, first strand synthesis was performed by incubating the pucks in RT solution for 1.5 hours at 52 C. RT solution: 115 μL H2O 40 μL Maxima 5x RT Buffer (Thermofisher, EP0751) 20 μL 10 mM dNTPs (NEB N0477L) 5 μL RNase Inhibitor (Lucigen 30281) 10 μL 50 μM Template Switch Oligo ( Qiagen #339414YCO0076714) 10 μL Maxima H-RTase (Thermofisher, EP0751)

Article Title: Profiling transcriptional heterogeneity with Seq-Well S 3 : A low-cost, portable, high-fidelity platform for massively-parallel single-cell RNA-seq
Article Snippet: Maxima 5x RT buffer. (Thermo-Fisher Cat. No EP0753).

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5x maxima rt buffer (Thermo Fisher)

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